Description
DNA methylation at CpG islands in promoters regulates gene expression. It is a dynamic epigenetic process affected by various genetic and environmental factors. Numerous diseases including cancer have been linked to aberrant global changes in genomic DNA methylation. It is generally thought that global genomic DNA methylation level can be represented by the methylation level at repetitive elements. One example of this includes the repetitive Long Interspersed Nuclear Element-1 (LINE-1), which compromises 17-18% of the human genome and is usually heavily methylated in most normal primary cells. ScienCell's Human Global DNA Methylation Quantification qPCR Assay Kit (HGDM) is designed to quantify the level of human LINE1 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated LINE1 promoter copies to non-methylated LINE1 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated LINE1 promotor of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency